This class covers transgenic technologies developed for model organisms including Drosophila, mammals, zebrafish, marine invertebrates, plants, fungi, and single-celled eukaryotes. The emphasis is on recent advances and on broadly applicable principles and tools that are successful across a wide range of eukaryotic taxa. The class combines lectures with the reading of original literature, and in-class and online discussions. The course is intended for advanced undergraduate and graduate students. Prerequisite: BIS101 (Genes and Genomes) or equivalent. Considerable background in general and molecular genetics on the part of the students is assumed. Class meets once a week for two hours.