Safety Documents
Trizol SOP (READ FOR RNA EXTRACTION)
Reproductive toxin SOP
Carcinogen SOP
Acid SOP
SOP for acutely toxic substances
Basic Molecular Biology
Cloning protocols
Bacterial stabbing and plate streaking
How to make electrocompetent cells
How to electroporate bacteria
Transformation using chemically competent cells (video link here)
Culturing transformed bacteria
Making LB broth or LB agar plates
Pouring LB plates
Nucleic Acid Extraction & Quantification
Trizol RNA purification for small amounts of tissue
Addendum to Trizol RNA extraction protocol
Single fly squish prep
Single fly genomic DNA extraction
Single fly DNA extraction with Qiagen Gentra Puregene kit
DNA prep from 20 flies
Phenol chloroform purification
Using the nanodrop
Sanger Sequencing
Sanger sequencing
Sanger sequencing cleanup
Drosophila germline transformation
Injection protocol (Gompel lab)
Fly protocols
Abdominal cuticle prep
Pupal collection times
Fixing fly pupae for legs
Oenocyte dissection
Immunohistochemistry
Whole mount immunohistochemistry protocol
Epidermis immunohistochemistry #1
Epidermis immunohistochemistry #2
In-situ hybridization protocols
DIG labeling of RNA probes #1
DIG labeling of RNA probes #2
DIG labeling of RNA probes #3
Pupal RNA in situ hybridization
Abdominal RNA in situ hybridization
Adult internal structures in situ hybridization
Imaginal disc in situ hybridization
Scanning electron microscopy
Preparing SEM sample
Imaging SEM protocol