A new paper in Nature Methods describes a simplified approach for generating and assembling RAD libraries. From the paper:
“Here we describe a streamlined and flexible approach for RAD genotyping, called 2b-RAD for its use of type IIB restriction enzymes. These enzymes (for example, BsaXI and AlfI) cleave genomic DNA upstream and downstream of the target site, producing tags of uniform length that are ideally suited for sequencing on existing next-generation platforms.”
Check it out.